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Analysis of proteins associated with storage root formation in cassava using two-dimensional gel electrophoresis Rev. Bras. Fisiol. Veg.
CABRAL,GLAUCIA B.; CARVALHO,LUIZ J.C.B..
Two-dimensional gel electrophoresis analysis was performed on adventitious and storage root in cassava (Manihot esculenta Crantz). Adventitious root lacking swelling formation and swelled storage root were obtained from the accession WU104 grown in the green house of the Department of Biology in Washington University in St. Louis (MO-USA). Saline buffer-soluble proteins were extracted, separated in a high-resolution 2-D electrophoresis system, visualized with silver staining gel procedure, and digital image generate for further analysis. Quantitative and qualitative protein spots analysis was performed with a computer assisted image software system. Results revealed large variation in the complexity of the gel protein profile between the two root systems....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cassava; Manihot esculenta; Storage root; 2-D gel electrophoresis; Proteins.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100005
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Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics Anais da ABC (AABC)
CABRAL,GLAUCIA B.; CARNEIRO,VERA T.C.; GOMES,ANA CRISTINA M.M.; LACERDA,ANA LUIZA; MARTINELLI,ADRIANA P.; DUSI,DIVA M.A..
ABSTRACT Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an option to introduce agronomic important traits to B. brizantha cv. Marandu. In addition, it will open the possibility to study in vivo the function of candidate genes involved in the apomictic reproduction. The objective of this work was to evaluate peeled seeds, isolated embryo from mature seeds, embryogenic calluses and embryogenic cell suspensions, as target explant for genetic transformation via...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forage; Gus; HptII; Hygromycin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401789
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